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pep-10888 v1 CC-BY-SA-4.0

Abaloparatide: Tymlos bone-building drug for osteoporosis

A synthetic peptide that stimulates new bone growth in people with osteoporosis at high fracture risk; FDA-approved drug (brand name Tymlos).

statuscomputed targetPTH1R length44 aa refs2
status 2 / 5
prediction metrics openfold3-mlx 0.3.1
ipTM0.761
pTM0.658
avg pLDDT47.4
ranking score0.902
STRUCTURE · PEP-10888 × PTH1R
ranking0.902
target interface 4.5Å peptide drag rotate · ctrl+scroll zoom · right-click pan
openfold3-mlx 0.3.1 · mmCIF ↓ download
sequence44 aa
151015202530354044
AVSEHQLLHDK GKSIQDLRRRE LLEKLLXKLHA DLRTRGSGSSC
in the news 1 article
overview readme

What this is

Abaloparatide (brand name Tymlos; development code BA058) is an FDA-approved prescription medication used to build new bone in people with osteoporosis who are at high risk of fracture. It is a synthetic 34-amino-acid peptide designed to mimic a natural protein called parathyroid hormone-related protein (PTHrP), which the body uses to regulate bone and calcium. Unlike most osteoporosis drugs that slow bone loss, abaloparatide actively stimulates bone formation — it is one of only three "anabolic" (bone-building) agents approved by the FDA. The FDA approved it in April 2017 for postmenopausal women with osteoporosis at high fracture risk, and extended that approval to men with osteoporosis in 2022. The sequence stored in this card (44 residues, ending in GSGSSC) is longer than the canonical 34-residue approved drug; the C-terminal extension appears to derive from a construct used in a laboratory study and is not part of the commercial abaloparatide molecule, which ends at position 34.

History

The story of abaloparatide begins with the PTH type-1 receptor (PTH1R), which was established in the 1990s as a promising target for osteoporosis therapy. A key insight emerged from studying primary hyperparathyroidism: continuously elevated PTH drives net bone resorption and calcium release, but when PTH was given in brief daily pulses, bone formation actually exceeded resorption — the drug teriparatide (PTH 1–34) was built on this pulsatile-activation principle and approved in 2002.

Parathyroid hormone-related protein (PTHrP) is a separate hormone that acts on the same PTH1R receptor, but its normal physiological role is local and paracrine — guiding fetal bone and cartilage development, modulating breast and smooth-muscle tissue — rather than controlling systemic calcium. Early research showed that PTHrP(1–34) activated PTH1R with shorter-lived signaling than PTH(1–34), and produced less hypercalcemia at comparable doses in preclinical studies. Radius Health (then a partnership with Ipsen) engineered abaloparatide as an optimized analog of PTHrP(1–34) with amino acid substitutions tuned to enhance receptor affinity and pharmacokinetic stability.

The molecular basis for abaloparatide's distinct behavior was characterized by Hattersley and colleagues (Endocrinology, 2016): abaloparatide binds PTH1R with approximately 10-fold preference for the active RG conformation over the inactive R0 conformation, whereas teriparatide binds both conformations with roughly equal affinity. This conformational selectivity translates to more transient intracellular signals per injection and, hypothetically, a broader anabolic window with less osteoclast-activating follow-through.

The pivotal Phase 3 ACTIVE trial (Miller et al., JAMA 2016) randomized 2,463 postmenopausal women with osteoporosis across 28 sites in 10 countries over 18 months and formed the basis for FDA approval on April 28, 2017. The ACTIVExtend trial then examined sequential transition to alendronate after the abaloparatide course. A subsequent trial in men (ATOM) established the male indication approved in 2022. The original class boxed warning for osteosarcoma risk — based on rat carcinogenicity data shared with teriparatide — was removed by the FDA in 2022 after accumulated post-marketing surveillance in humans showed no osteosarcoma signal.

What it does

Abaloparatide stimulates bone-building cells (osteoblasts) to lay down new bone faster than bone-removing cells (osteoclasts) remove it. This is the opposite of how most osteoporosis medications work: bisphosphonates and denosumab mainly slow the removal of bone, while abaloparatide actively increases formation. The result is measurable bone density gains at the spine and hip, and a reduction in fracture risk, within months of starting treatment.

The key design feature is abaloparatide's preference for the active (RG) conformation of the PTH1R receptor. Binding this conformation produces a shorter burst of intracellular cAMP signaling per injection compared to teriparatide — a more transient pulse that drives osteoblast activity without the prolonged cAMP tail that tends to also activate osteoclasts and raise calcium. This mechanistic difference is hypothesized to explain the clinical observation that abaloparatide causes less transient hypercalcemia than teriparatide.

In the ACTIVE trial, abaloparatide produced mean lumbar spine bone mineral density (BMD) gains of approximately 11% from baseline at 18 months, versus 0.6% for placebo (Miller et al., JAMA 2016). Hip BMD gains were significantly faster than with teriparatide at 6 months. A notable property of all PTH1R anabolic agents is that BMD gains are largely reversed after discontinuation unless an antiresorptive drug (bisphosphonate or denosumab) is started to consolidate them — this sequencing is standard clinical practice.

Evidence

  • Human: Extensive. The pivotal ACTIVE Phase 3 trial (n=2,463 postmenopausal women, 18 months) showed an 86% reduction in new vertebral fractures versus placebo (0.58% vs 4.22%, P<0.001) and a 43% reduction in nonvertebral fractures (HR 0.57, P=0.049) (Miller et al., JAMA 2016). Hypercalcemia occurred in 3.4% of abaloparatide patients versus 6.4% with teriparatide (P=0.006). The ACTIVExtend trial showed that transitioning to alendronate after the abaloparatide course preserved and extended fracture-risk reduction over an additional 24 months. The ATOM trial established BMD gains and safety in men with osteoporosis, supporting the male indication approved in 2022. Multiple meta-analyses and network meta-analyses have examined abaloparatide in the broader context of available osteoporosis therapies.
  • Preclinical: Comprehensive. Ovariectomized-rat, orchiectomized-rat, and other animal models characterized abaloparatide's anabolic effect and confirmed the mechanistic RG-conformation selectivity story described by Hattersley and colleagues (Endocrinology, 2016).
  • In vitro: The RG vs R0 PTH1R binding-selectivity experiments used competitive radioligand binding and cAMP reporter assays in PTH1R-expressing HEK293 cells (Hattersley et al., Endocrinology 2016), establishing the molecular pharmacology that underlies the clinical differentiation from teriparatide.

Known effects

  • Reduction in vertebral fracture risk — FDA-approved (Phase 3 ACTIVE trial)
  • Reduction in nonvertebral fracture risk — FDA-approved
  • Bone mineral density gain at lumbar spine, total hip, femoral neck — FDA-approved; well-characterized in ACTIVE
  • Lower rate of treatment-emergent hypercalcemia vs teriparatide — Demonstrated in ACTIVE (3.4% vs 6.4%, P=0.006)
  • Bone-building effect in men with osteoporosis — FDA-approved (ATOM trial)
  • Preservation of BMD gains with sequential antiresorptive — Demonstrated in ACTIVExtend

Safety signals

Injection-site reactions were the most common adverse event in the ACTIVE trial (57.8% with abaloparatide vs 11.9% with placebo), predominantly mild. Nausea and dizziness occurred in approximately 10% each with abaloparatide. A transient increase in heart rate of approximately 8 beats per minute within one hour of dosing was observed; cardiovascular safety analysis from the ACTIVE trial did not identify increased serious cardiac events. Transient hypercalcemia was significantly less frequent with abaloparatide than with teriparatide (3.4% vs 6.4%, P=0.006 in ACTIVE). Orthostatic hypotension — a blood-pressure drop on standing — can occur shortly after injection, particularly after the first dose.

The original boxed warning for osteosarcoma risk (derived from Fischer 344 rat carcinogenicity studies at high doses) was removed by the FDA in 2022, alongside the equivalent teriparatide warning, based on accumulated post-marketing surveillance data showing no human osteosarcoma signal. Labeled contraindications for patients at baseline elevated osteosarcoma risk — those with Paget's disease of bone, prior skeletal radiation therapy, unexplained elevated alkaline phosphatase, or open bone growth plates (pediatric or young adult patients) — remain in force as class-level cautions.

Regulatory status

  • US: FDA-approved prescription drug (Tymlos). Initial approval April 28, 2017 for postmenopausal women with osteoporosis at high fracture risk; extended to men with osteoporosis in 2022. The original boxed warning for osteosarcoma and a prior cumulative lifetime use restriction were removed by the FDA in 2022. Not a controlled substance; distributed through specialty pharmacies.
  • EU: The original EMA marketing authorization application was withdrawn following a negative CHMP opinion in 2018. Subsequently approved by the UK MHRA in 2022 and available in several other European markets via national pathways or subsequent applications.
  • Canada: Health Canada approved.
  • WADA: Abaloparatide is not specifically named on the WADA Prohibited List; its anabolic bone pharmacology and PTH1R-axis activity would draw doping-program scrutiny for performance-related off-label use.

Myths and misconceptions

  • "Abaloparatide is just a modified teriparatide and works the same way" — While both activate PTH1R, the conformational selectivity difference produces clinically relevant distinctions: abaloparatide causes significantly less hypercalcemia (3.4% vs 6.4%, P=0.006 in ACTIVE) and showed faster hip BMD gains at 6 months. Abaloparatide is also a PTHrP-derived analog, not a PTH-derived one — a different parent peptide engineered to a different receptor binding profile. It is a brand-name drug (Tymlos), not a biosimilar or generic.
  • "Two years of abaloparatide will permanently build bone" — BMD gains are substantially reversed after stopping unless an antiresorptive agent is started. The ACTIVExtend trial was designed specifically to demonstrate the sequential anabolic-then-antiresorptive paradigm: patients who transitioned to alendronate after abaloparatide maintained fracture-risk reduction through the antiresorptive period; stopping without the antiresorptive bridge allows gains to erode.
  • "Abaloparatide causes osteosarcoma in humans" — The original boxed warning derived from rat carcinogenicity studies at high lifetime doses. Post-marketing surveillance following years of teriparatide use (approved 2002) and abaloparatide use identified no human osteosarcoma signal. The FDA removed the boxed warning and cumulative use restriction in 2022. Class-specific baseline-risk contraindications (Paget's disease, prior bone radiation, open growth plates) remain.
  • "Abaloparatide is safer than teriparatide overall" — The lower hypercalcemia rate is real and mechanistically grounded, but the two drugs share the same class cautions, overlapping adverse-event profiles, and broadly similar safety considerations. The choice between them in clinical practice typically involves access, cost, injection-device preferences, and individual patient calcium-monitoring context rather than a blanket safety advantage for either.

Common questions

How does abaloparatide compare to romosozumab (Evenity)? Romosozumab is an anti-sclerostin monoclonal antibody that works by a distinct mechanism — it inhibits sclerostin, unlocking the Wnt/β-catenin pathway to simultaneously increase bone formation and reduce resorption. Unlike abaloparatide's daily injection schedule, romosozumab is given monthly for 12 months. The ARCH trial showed romosozumab followed by alendronate reduced vertebral fractures more than alendronate alone; romosozumab's label carries a cardiovascular warning (increased risk in patients with prior stroke or MI). No head-to-head randomized trial has compared abaloparatide and romosozumab directly on fracture outcomes.

Can abaloparatide be used in men with osteoporosis? Yes. The ATOM trial enrolled men with osteoporosis and showed BMD gains consistent with results in women from the ACTIVE trial. The FDA extended the Tymlos indication to include men in 2022.

Is there a non-injection form of abaloparatide? A transdermal microneedle-patch formulation (abaloparatide-sMTS) was evaluated in a Phase 3 trial but did not meet non-inferiority versus subcutaneous injection for the primary BMD endpoint. Development of this route continues; the only currently approved formulation is subcutaneous injection.

Related peptides

  • Teriparatide — PTH(1–34); the first PTH1R anabolic agent for osteoporosis (approved 2002); binds both RG and R0 receptor conformations with similar affinity; associated with higher rates of transient hypercalcemia compared to abaloparatide in the ACTIVE trial
  • BPC-157 — a synthetic peptide studied for tissue repair and gastric protection; mechanistically unrelated to PTH1R signaling
Hypotheses3 directions▾ collapse

Research directions for this peptide, selected from the current sources — hypotheses you can explore and model. None of it is proven yet; tap any one to see the full thinking.

openupdated 2026-06-05

If abaloparatide is placed directly at a fracture site rather than injected into the bloodstream, could it stimulate faster bone repair without raising dangerous calcium levels?

This could provide surgeons with a tool to accelerate healing of difficult fractures in osteoporotic patients, reducing the rate of failed fixation, repeat surgeries, and disability in an aging population.

The hypothesis
Local delivery of abaloparatide from a biodegradable hydrogel carrier at fracture sites produces faster cortical bridging than systemic subcutaneous injection at equivalent total doses, because PTH1R activation in periosteal osteoblasts is concentration-dependent and local delivery achieves receptor-saturating concentrations at the fracture gap that systemic dosing cannot without causing hypercalcemia.
Why it’s plausible
The literature snippet documents a 3D gelatin hydrogel abaloparatide delivery experiment that produced continuous local release. Systemic teriparatide accelerates fracture healing in clinical use, but dose is limited by hypercalcemia risk. Local delivery bypasses systemic calcium effects while achieving high periosteal concentrations. Abaloparatide has lower hypercalcemic risk than teriparatide systemically, but local delivery could exploit this further.
Why it matters
This would establish a new indication for abaloparatide, fracture augmentation at the time of surgical repair, addressing a large unmet need in delayed union and non-union fractures, particularly in osteoporotic bone where standard fixation has high failure rates.
Plausibility.65
Novelty.55
Impact.70
Basis · grounding2 papers · 1 computed/note
[1]
paper
Abaloparatide loaded into methacrylate gelatin hydrogel for continuous local delivery; bone effects studied
doi: 10.1016/j.ejphar.2021.174409
[2]
paper
Systemic abaloparatide reduces fracture risk in postmenopausal osteoporosis; bone formation marker changes confirm anabolic effect
doi: 10.1001/jama.2016.11136
[3]
sourceAbaloparatide plasma clearance and PK data; short systemic half-life suggests local delivery reservoir would provide prolonged periosteal exposure beyond systemic
openupdated 2026-06-05

Does abaloparatide lock onto its receptor in a particular shape that stimulates bone building while avoiding the bone-breakdown signal that limits other drugs?

Understanding this mechanism could explain why abaloparatide builds bone faster than teriparatide, and could guide the design of even better osteoporosis drugs that build bone without the two-year safety limit imposed on current treatments.

The hypothesis
Abaloparatide preferentially stabilizes the PTH1R receptor in the R0 conformation (G protein-uncoupled, high-affinity state) rather than the RG conformation (G protein-coupled), which accounts for its anabolic-to-antiresorptive activity ratio exceeding that of teriparatide, because R0-selective engagement promotes transient cAMP bursts in osteoblasts without sustained activation of bone-resorbing osteoclast coupling signals.
Why it’s plausible
The PTH1R literature distinguishes R0 (receptor alone) from RG (receptor-G protein complex) conformations, with different ligand preferences. Teriparatide stabilizes RG, producing prolonged cAMP signals and coupled resorption. Abaloparatide was designed to have shorter receptor occupancy and more anabolic selectivity. The ipTM of 0.76 against PTH1R suggests a well-defined but potentially non-canonical binding mode.
Why it matters
Confirming R0 selectivity as the mechanism for abaloparatide's superior anabolic-to-resorptive ratio would provide a rational design principle for next-generation PTH1R agonists that maximize bone formation while minimizing the resorptive coupling that limits the 2-year treatment window of current anabolic agents.
Plausibility.65
Novelty.40
Impact.70
Basis · grounding1 paper · 2 computed/notes
[1]
sourcePTH1R R0 vs. RG conformation pharmacology reviewed; receptor conformational states as drug design targets
[2]
paper
Abaloparatide dose-response data showing distinct potency profile from teriparatide at PTH1R
doi: 10.1210/en.2015-1726
[3]
structureipTM=0.76 for PTH1R complex: well-defined binding, consistent with selective receptor conformation engagement
openupdated 2026-06-05

Is the mysterious position-26 residue in abaloparatide a helix-stiffening modification that makes the peptide latch onto its bone receptor faster and more strongly?

Identifying this key structural feature would provide a blueprint for engineering the next generation of bone-building drugs with even greater potency, potentially helping patients who do not respond adequately to current treatments.

The hypothesis
The unknown residue at position 26 (stored as 'X') in abaloparatide's sequence AVSEHQLLHDKGKSIQDLRRRELLEKLLXKLHADLRTR corresponds to alpha-aminoisobutyric acid (Aib), and this helix-constraining non-standard residue is the primary determinant of abaloparatide's superior helical stability and faster receptor on-rate relative to teriparatide, whose corresponding position carries Leu26.
Why it’s plausible
Abaloparatide was developed using structure-inducing probe (SIP) technology, which systematically incorporates Aib residues to constrain peptide helices. The readme describes abaloparatide as a PTHrP analog incorporating helix-stabilizing modifications. Position 26 in PTHrP-related peptides corresponds to a hydrophobic position in the amphipathic helix; Aib at this position would cap the helix and reduce conformational entropy of binding. The stored 'X' is a placeholder for a non-standard residue, consistent with Aib.
Why it matters
If Aib26 is confirmed as the key helix-stabilizing residue, it would validate the SIP engineering strategy for bone-anabolic peptides and identify a specific substitution transferable to next-generation PTH1R agonists aimed at improving activity-duration ratios beyond the current clinical candidates.
Plausibility.75
Novelty.35
Impact.55
Basis · grounding1 paper · 2 computed/notes
[1]
sequenceAVSEHQLLHDKGKSIQDLRRRELLEKLLXKLHADLRTR: 'X' at position 26 is a non-standard residue; Aib is the canonical SIP modification
[2]
paper
Structure-inducing probe (SIP) technology used in lixisenatide development (same methodology underpins abaloparatide design); Aib residues are the SIP modification
doi: 10.1124/pr.115.011395
[3]
noteAbaloparatide incorporates helix-stabilizing modifications designed to improve receptor binding; SIP technology mentioned in historical development
details expand to inspect
full evidence table2 metrics
metricvaluetool
ipTM 0.7607945799827576 openfold3-mlx
ranking score 0.9019421339035034 openfold3-mlx
structural qualityopenfold3
0
metricvaluenote
gpde0.782global PDE — lower = better
disorder0.323! high disorder
chain pair ipTM (A, B)0.761interface quality
3-letter notation
Ala-Val-Ser-Glu-His-Gln-Leu-Leu-His-Asp-Lys-Gly-Lys-Ser-Ile-Gln-Asp-Leu-Arg-Arg-Arg-Glu-Leu-Leu-Glu-Lys-Leu-Leu-X-Lys-Leu-His-Ala-Asp-Leu-Arg-Thr-Arg-Gly-Ser-Gly-Ser-Ser-Cys
recipeopenfold3-mlx 0.3.1
parametervalue
modelopenfold3-mlx 0.3.1
weights
hardware
mlx version
python
random seed
msa strategy
diffusion samples1
runtime835s
predicted bymlx@peptide
predicted at2026-05-03
citationbibtex
peptidemodel (2026). Abaloparatide: Tymlos bone-building drug for osteoporosis (pep-10888, v1). PeptideModel. https://peptidemodel.com/card/pep-10888
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  sequence = {AVSEHQLLHDKGKSIQDLRRRELLEKLLXKLHADLRTRGSGSSC},
  target   = {pth1r},
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}
related peptides 5 by signal overlap
clinical trials 1 on ct.gov · checked 2026-05-22
ct.gov trials 1
by phase
1phase 1
by status
1completed
references 2 papers
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