pe
pep-10735 v1 CC-BY-SA-4.0

IGF-II growth factor fragment (IGF-II 33-40)

A tiny piece of IGF-II, a natural body growth factor; studied in the lab for how it interacts with growth signals, research tool only, not an approved drug.

statusbioassayed targetIGF-1R length8 aa refs8
status 4 / 5 · 2 verified on platform
prediction metrics boltz-2 1.0
ipTM0.280
pTM0.360
avg pLDDT67.3
ranking score0.594
STRUCTURE · PEP-10735 × IGF-1R
ranking0.594
target interface 4.5Å peptide drag rotate · ctrl+scroll zoom · right-click pan
boltz-2 1.0 · mmCIF ↓ download
sequence8 aa
158
SRVSRRSR
in the news 1 article
overview readme

What this is

IGF-II (33-40) is an eight-amino-acid fragment of insulin-like growth factor II (IGF-II), a small protein hormone that the body makes naturally. The stored sequence here, SRVSRRSR, corresponds to a short stretch of residues from the middle of the parent IGF-II molecule rather than the full hormone. IGF-II itself is one of two insulin-like growth factors (the other being IGF-I) that signal mainly through the type 1 IGF receptor (IGF-1R) and play a central role in growth, tissue repair, and metabolism (LeRoith 2021).

This card describes a short peptide fragment derived from that larger hormone. It should not be conflated with intact IGF-II or with IGF-I — the biology summarised below comes from work on the full-length ligands and on the IGF-1R receptor, because that is what the dossier supports. Fragment-specific evidence is sparse.

What it does

In its full-length form, IGF-II binds to the same receptor used by IGF-I — the type 1 insulin-like growth factor receptor (IGF-1R), a transmembrane tyrosine kinase. Ligand binding relaxes conformational restraints in the receptor's dimeric ectodomain and triggers transphosphorylation of the intracellular kinase domains, launching downstream signalling that drives cell growth, differentiation, and survival (Xu 2018, Girnita 2014). IGF-1R is closely related to the insulin receptor and the two can heterodimerise, with their isoform composition shaping the metabolic versus mitogenic balance of the response (Belfiore 2017).

What the isolated SRVSRRSR fragment does on its own — whether it binds the receptor, modulates IGF binding-protein interactions, or has any independent activity — is not documented in the sources gathered for this card.

Mechanism

The IGF-1R is a disulfide-linked α₂β₂ homodimer in the receptor tyrosine kinase family. A single IGF molecule engages the receptor across both half-sites of the ectodomain; binding shifts the autoinhibited dimer into an active configuration that allows the two intracellular kinase domains to transphosphorylate each other on activation-loop tyrosines (Xu 2018). The activated receptor then recruits IRS adaptors and Shc, feeding into the PI3K–Akt and Ras–MAPK pathways that mediate the growth, anti-apoptotic, and proliferative effects of IGF signalling (Girnita 2014).

Circulating IGFs do not travel free in plasma — they are carried by a family of IGF binding proteins (IGFBPs) that regulate ligand availability at target tissues. Engineered IGF-I variants that bind IGFBPs poorly, such as des(1-3)IGF-I and Long-R3-IGF-I (LR3-IGF-I, with an N-terminal extension and an Arg substitution at position 3), are more potent in vivo than native IGF-I because more of the injected dose reaches the receptor (Tomas 1993). The same logic underlies the increased delivery of LR3-IGF-I from blood into extracellular wound-fluid sites compared with native IGF-I, where IGFBPs slow endothelial transit (Bastian 2000). These observations describe the parent ligands, not the SRVSRRSR fragment.

History

IGF-I and IGF-II were characterised in the 1970s as the "somatomedin" activities mediating many of growth hormone's effects on tissue growth. Decades of work since have mapped the IGF axis — ligands, six high-affinity IGFBPs, and the IGF-1R and IGF-2R receptors — into one of the better-studied endocrine systems, with clinical interest spanning growth disorders, diabetes, ageing, and oncology (LeRoith 2021, Fernández-Garza 2025). The dossier does not contain a primary record of when or by whom the IGF-II (33-40) fragment specifically was first synthesised or studied.

Evidence

  • Human: No human trials of the SRVSRRSR fragment itself are present in the dossier. A first-in-human study of a PEGylated recombinant full-length human IGF-I has been reported (Kletzl 2017), and a clinical review summarises growth hormone and IGF-1 use in aging contexts (Fernández-Garza 2025) — both relate to parent IGF ligands rather than this fragment.
  • Animal: Rat work on full-length IGF-I and on the LR3-IGF-I variant has shown dose-dependent growth and wound-fluid delivery effects (Tomas 1993, Bastian 2000). No animal data on IGF-II (33-40) is present in the dossier.
  • In vitro: Structural and signalling work on IGF-1R itself (Xu 2018) and on receptor regulation (Girnita 2014, Belfiore 2017) provides the receptor-side picture. The dossier contains no in vitro characterisation of the isolated SRVSRRSR fragment.

Open questions

  • Whether the isolated SRVSRRSR octapeptide binds IGF-1R, IGFBPs, or any other target with measurable affinity.
  • Whether it has agonist, antagonist, or no activity at the IGF-1R, given that IGF-1R binding by the parent ligand depends on multiple contact regions distributed across the IGF molecule (Xu 2018).
  • Pharmacokinetics, proteolytic stability, and tissue distribution of the fragment have not been reported in the sources reviewed.
  • Whether the fragment can serve as a tool reagent (e.g. as a competitive probe for parent-ligand contacts) or has any therapeutic relevance is not addressed in the dossier.

Regulatory status

  • US/EU: Not an approved drug. IGF-II (33-40) is a research peptide fragment; no regulatory authorisation is recorded.
  • WADA: Full-length IGF-1 and related growth factors fall under WADA's prohibited list (S2, peptide hormones, growth factors and related substances). The status of short IGF-II fragments is not separately addressed in the dossier sources; treat parent-ligand restrictions as the relevant policy frame for context, not as a verdict on this specific fragment.

Related peptides

  • IGF-I and its engineered variants des(1-3)IGF-I and LR3-IGF-I — used in the rat studies above to probe how IGFBP binding shapes in vivo potency and tissue delivery (Tomas 1993, Bastian 2000).
  • PEGylated recombinant human IGF-I — tested in a first-in-human pharmacokinetic study (Kletzl 2017).
  • Full-length IGF-II — the parent hormone from which this fragment is derived.

Internal cross-reference links to other Peptidopedia cards have been omitted because the relevant pep-ids could not be verified from the dossier.

Hypotheses4 directions▾ collapse

Research directions for this peptide, selected from the current sources — hypotheses you can explore and model. None of it is proven yet; tap any one to see the full thinking.

openupdated 2026-06-05

Is this peptide clinging to its target mainly because it is highly charged, rather than fitting precisely?

If true, chemists would know to redesign the peptide with a better balance of charges so it binds more specifically. This could lead to drugs that hit only the intended target and cause fewer side effects.

The hypothesis
The arginine-rich motif in SRVSRRSR (positions 3, 5, 6, 8 are arginine) drives non-specific electrostatic adhesion to IGF-1R rather than specific recognition, and substituting one or two arginines with neutral residues would abolish binding without losing fragment stability.
Why it’s plausible
The sequence SRVSRRSR contains four arginine residues in an 8-mer, creating a highly cationic patch. The low ipTM suggests this may be driven by non-specific electrostatic attraction to acidic patches on the IGF-1R ectodomain rather than shape complementarity. Structure predictions of arginine-to-alanine variants could test whether the positive charge cluster is the primary driver of the weak interaction.
Why it matters
If the interaction is primarily electrostatic, this fragment is unlikely to be a specific therapeutic lead. However, understanding this would prevent wasted development effort and guide rational design toward more selective charge-balanced analogs.
Plausibility.70
Novelty.35
Impact.40
Basis · grounding2 computed/notes
[1]
sequenceSRVSRRSR contains arginines at positions 3, 5, 6, and 8 (R3, R5, R6, R8), creating a highly basic 8-amino-acid peptide with net charge +4 at physiological pH
[2]
structureLow ipTM (0.28) for the IGF-1R complex is consistent with non-specific electrostatic adhesion rather than high-specificity binding
openupdated 2026-06-05

Could a very short piece of IGF-II stick to its receptor in an unexpected place?

If true, doctors might one day use ultra-small peptides to gently dial growth signals up or down, instead of flooding the body with large hormones. This could mean safer treatments for children with growth disorders or adults with certain cancers.

The hypothesis
The SRVSRRSR fragment binds IGF-1R at a site distinct from the full-length IGF-II binding pocket, acting as a weak allosteric modulator rather than a competitive agonist.
Why it’s plausible
The fragment spans residues 33-40 of IGF-II, which falls within the C-domain region known to differ between IGF-I and IGF-II in receptor interactions. The low ipTM (0.28) and moderate pLDDT (67.3) from the structure prediction suggest the fragment makes only partial or transient contact with IGF-1R, inconsistent with a high-affinity orthosteric binding mode. The C-domain of IGF-II has been implicated in receptor specificity, suggesting this short stretch may contact a secondary surface.
Why it matters
If true, this would reveal that even short internal fragments of IGF-II can engage the receptor through non-canonical interfaces, opening a path to design minimal allosteric peptides that modulate IGF-1R without mimicking the full growth factor.
Plausibility.55
Novelty.50
Impact.45
Basis · grounding1 paper · 2 computed/notes
[1]
structureBoltz-2 complex prediction: ipTM=0.2799915671348572, pLDDT=67.3 for SRVSRRSR with IGF-1R, indicating weak/partial interface confidence
[2]
paper
IGF-II C-domain chimera (IGF-I CII containing the shorter C-domain of IGF-II) shows altered binding to IGF-1R, demonstrating the C-domain modulates receptor engagement
doi: 10.1038/s41467-018-03219-7
[3]
noteFragment spans residues 33-40, within the C-domain region of IGF-II that differs from IGF-I and influences receptor interactions
openupdated 2026-06-05

Could this short fragment stick to the insulin receptor just as strongly as to its intended target?

If true, drug developers would know early that this peptide is not selective enough on its own. They could then engineer in extra specificity before spending years on development, avoiding drugs that accidentally trigger blood sugar crashes or unwanted cell growth.

The hypothesis
SRVSRRSR binds the insulin receptor (IR-A) with comparable or higher affinity than IGF-1R, because the fragment lacks the C-domain determinants that confer IGF-1R selectivity in full-length IGF-II, and the arginine cluster matches a conserved acidic patch on both receptors.
Why it’s plausible
Full-length IGF-II binds both IGF-1R and IR-A, with relative affinities shaped by the C-domain. The fragment SRVSRRSR is drawn from the C-domain region but lacks the larger structural context that tunes receptor selectivity. If the fragment-receptor interaction is dominated by the arginine cluster, it may not discriminate between the two closely related receptors. The IGF-1R/IR-A heterodimerization noted in the readme further complicates selectivity.
Why it matters
If true, any therapeutic development based on this fragment would need to account for insulin receptor cross-talk, which could cause metabolic side effects (hypoglycemia) or unintended mitogenic signaling through IR-A isoforms in cancer.
Plausibility.50
Novelty.40
Impact.55
Basis · grounding1 paper · 2 computed/notes
[1]
paper
IGF-2 V43M analog binds M6P/IGF-2R with high affinity but IR-A and IGF-1R with very low affinity, showing receptor selectivity is encoded in specific residues outside this fragment
doi: 10.1210/er.2017-00073
[2]
noteIGF-1R and insulin receptor can heterodimerise, with isoform composition shaping metabolic versus mitogenic responses; the fragment lacks the structural elements that tune this balance
[3]
sequenceSRVSRRSR is a short arginine-rich fragment without the larger IGF-II structural scaffold that normally confers receptor binding specificity
openupdated 2026-06-05

Could this peptide find tumors simply because it carries a strong positive charge?

If true, it could be attached to chemotherapy drugs like a guided missile, carrying toxic payloads straight to tumors while sparing healthy tissue. Cancer patients could receive stronger treatments with fewer side effects.

The hypothesis
The cationic arginine-rich surface of SRVSRRSR enables cell-penetrating or membrane-associating activity independent of IGF-1R binding, and the fragment may accumulate in acidic tumor microenvironments or negatively charged extracellular matrix regions through electrostatic targeting.
Why it’s plausible
Peptides with four arginines in eight residues are strongly cationic and often exhibit cell-penetrating properties (e.g., Tat-derived peptides, poly-arginine transduction domains). The low ipTM with IGF-1R suggests the primary biological activity of this fragment may not be receptor-mediated. Tumor microenvironments and injured tissues often present acidic surfaces and negatively charged matrix components that attract cationic peptides.
Why it matters
If true, this fragment could be repurposed as a tumor-targeting delivery vehicle or a probe for diseased tissue, even if it has no direct IGF-1R pharmacology. The IGF-II origin provides a secondary targeting rationale for IGF-1R-expressing cancers.
Plausibility.55
Novelty.30
Impact.50
Basis · grounding1 paper · 2 computed/notes
[1]
sequenceSRVSRRSR has a net charge of approximately +4 at physiological pH due to four arginine residues in an 8-mer, a hallmark of cell-penetrating and membrane-associating peptides
[2]
structureLow ipTM (0.28) with IGF-1R suggests the fragment may have weak specific receptor binding, pointing to alternative biological roles mediated by its physicochemical properties
[3]
paper
IGF-1R targeting suppresses rhabdomyosarcoma growth, establishing the therapeutic relevance of IGF-axis biology in cancer contexts where this fragment might accumulate
doi: 10.1007/s00018-013-1514-y
details expand to inspect
full evidence table2 metrics
metricvaluetool
ipTM 0.2799915671348572 boltz-2
ranking score 0.5943511724472046 boltz-2
structural qualityopenfold3
metricvaluenote
gpde1.983global PDE — lower = better
disorderNaNfraction disordered
3-letter notation
Ser-Arg-Val-Ser-Arg-Arg-Ser-Arg
recipeboltz-2 1.0
parametervalue
modelboltz-2 1.0
weights
hardwarenvidia_nim_api
mlx version
python
random seed
msa strategynone
diffusion samples1
runtime
predicted bymlx@peptide
predicted at2026-04-24
citationbibtex
peptidemodel (2026). IGF-II growth factor fragment (IGF-II 33-40) (pep-10735, v1). PeptideModel. https://peptidemodel.com/card/pep-10735
@peptide{pep10735,
  sequence = {SRVSRRSR},
  target   = {igf-1r},
  author   = {peptidemodel},
  year     = {2026},
  status   = {bioassayed}
}
clinical trials 254 on ct.gov · 7 on EUCTR · checked 2026-05-22
ct.gov trials 254
with results 65
EUCTR 7
PubMed RCT 13
by phase
5phase 22phase 34no phase
by status
8completed1active1not yet recruiting
references 8 papers
discussion no comments
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peptidemodel.com CC-BY-SA-4.0 research only · not for human use