pe
pep-10633 v1 CC-BY-SA-4.0

Substance P (1-7): nerve-signaling fragment that lowers blood pressure and eases opioid withdrawal

A naturally occurring fragment of substance P, a pain and inflammation messenger; lowers blood pressure and slows heart rate in the brain's cardiovascular control center, and may reduce opioid withdrawal signs in mice, used only as a lab research tool.

statusbioassayed targetTACR1 length7 aa refs2
snapshot sparse 0% confidence
Class
Endogenous neuropeptide / tachykinin
Status
No approved therapeutic status identified in attached sources
Main caveat
Card written from catalog and overview text only (SB + CU sources); no study, trial, or assay data are individually attached.
status 4 / 5 · 2 verified on platform
prediction metrics boltz-2 1.0
ipTM0.910
pTM0.776
avg pLDDT69.5
ranking score0.738
STRUCTURE · PEP-10633 × TACR1
ranking0.738
target interface 4.5Å peptide drag rotate · ctrl+scroll zoom · right-click pan
boltz-2 1.0 · mmCIF ↓ download
sequence7 aa
157
RPKPQQF
in the news 1 article
overview readme

What this is

Substance P (1-7) is a naturally occurring fragment of substance P, a well-known signaling peptide found throughout the nervous and immune systems. It consists of the first seven amino acids of the full 11-residue substance P sequence — the same N-terminal stretch that anchors substance P to its primary receptor. Researchers use this fragment to probe how the NK1 receptor (also called the neurokinin-1 receptor) recognizes its ligand, and to tease apart which part of substance P is responsible for which biological effects.

What it does

In the cardiovascular and autonomic nervous system, Substance P (1-7) has been reported to lower blood pressure and slow heart rate when acting in the nucleus tractus solitarius, a brainstem region that integrates cardiovascular control signals. Separately, studies in mice have found that this fragment can reduce signs of opioid withdrawal, suggesting it modulates pain and stress circuitry independently of its role in the immune system.

At the receptor level, substance P and the NK1 receptor are a well-characterized signaling pair. When substance P (or fragments that retain receptor binding) engages NK1R — a G protein-coupled receptor — this triggers activation of NF-κB and the release of proinflammatory cytokines, as reviewed by Douglas and colleagues (Annals of the New York Academy of Sciences, 2011). NK1R itself exists in two forms: a full-length isoform and a truncated isoform, which differ in their functional significance (Douglas et al. 2011).

Evidence

  • Human: No human clinical trials for Substance P (1-7) specifically are known from published literature.
  • Animal: Depressor and bradycardic effects have been reported in animal models following administration into the nucleus tractus solitarius; opioid withdrawal signs reduced in mice.
  • In vitro: NK1R binding and downstream NF-κB/cytokine signaling characterized in cellular models (Douglas et al. 2011).

Mechanism

Substance P (1-7) retains the N-terminal sequence RPKPQQF from the full-length peptide RPKPQQFFGLM-NH2. The NK1 receptor (tacr1) is the primary target; binding to NK1R couples through G proteins to activate NF-κB and downstream proinflammatory cytokines (Douglas et al. 2011). Schank and colleagues (International Review of Neurobiology, 2017) have framed the SP/NK1R system as a stress-response pathway with functional parallels to corticotropin-releasing factor (CRF) signaling — a framework that helps contextualize Substance P (1-7)'s reported effects on autonomic cardiovascular tone and opioid-related behavior.

NK1R is ubiquitous across species and tissues. Its two isoforms — full-length and truncated — have distinct functional profiles (Douglas et al. 2011), a distinction relevant to interpreting receptor binding studies with short fragments such as SP(1-7).

Related peptides

  • Substance P (full-length, RPKPQQFFGLM-NH2) is the parent peptide from which this fragment derives; the C-terminal residues FGLM are absent in SP(1-7), differentiating their receptor pharmacology.
Hypotheses1 direction▾ collapse

Research directions for this peptide, selected from the current sources — hypotheses you can explore and model. None of it is proven yet; tap any one to see the full thinking.

openupdated 2026-06-11

Could this fragment calm withdrawal symptoms by acting on a different target in the brain stem?

If confirmed, it could point to an entirely new drug strategy for opioid withdrawal that avoids the inflammatory risks of activating the standard substance P receptor. This matters for the millions of people seeking safer withdrawal treatments.

The hypothesis
Substance P (1-7) suppresses opioid withdrawal signs through a NK1R-independent mechanism involving direct modulation of brainstem autonomic circuits in the nucleus tractus solitarius, separate from classical neurokinin signaling.
Why it’s plausible
The README reports SP(1-7) reduces opioid withdrawal signs in mice and lowers blood pressure when acting in the NTS. The NTS integrates autonomic and stress signals. Full SP and NK1R agonism typically increase stress and pain signaling, not suppress them. If SP(1-7) anti-withdrawal activity were purely NK1R-mediated it would be expected to worsen, not attenuate, withdrawal. This implies an alternative target or mechanism in the NTS.
Why it matters
Identifying a non-NK1R autonomic mechanism for SP(1-7) would reveal a new pathway for treating opioid withdrawal without engaging the proinflammatory NK1R axis, reducing risk of immune-related side effects.
Plausibility.75
Novelty.45
Impact.70
Basis · grounding1 paper · 1 computed/note
[1]
noteREADME states SP(1-7) reduces opioid withdrawal signs in mice and lowers blood pressure/heart rate in the NTS
[2]
paper
NK1R antagonism attenuates stress-induced cortisol, suggesting NK1R activation typically drives stress; SP(1-7) anti-stress effects would therefore be paradoxical if NK1R-mediated
doi: 10.1016/bs.irn.2017.06.008
details expand to inspect
full evidence table2 metrics
metricvaluetool
ipTM 0.9104166626930237 boltz-2
ranking score 0.7384291887283325 boltz-2
structural qualityopenfold3
metricvaluenote
gpde1.480global PDE — lower = better
disorderNaNfraction disordered
3-letter notation
Arg-Pro-Lys-Pro-Gln-Gln-Phe
recipeboltz-2 1.0
parametervalue
modelboltz-2 1.0
weights
hardwarenvidia_nim_api
mlx version
python
random seed
msa strategynone
diffusion samples1
runtime
predicted bymlx@peptide
predicted at2026-04-24
citationbibtex
peptidemodel (2026). Substance P (1-7): nerve-signaling fragment that lowers blood pressure and eases opioid withdrawal (pep-10633, v1). PeptideModel. https://peptidemodel.com/card/pep-10633
@peptide{pep10633,
  sequence = {RPKPQQF},
  target   = {tacr1},
  author   = {peptidemodel},
  year     = {2026},
  status   = {bioassayed}
}
related peptides 3 by signal overlap
clinical trials 1103 on ct.gov · 47 on EUCTR · checked 2026-05-09
ct.gov trials 1103
with results 244
EUCTR 47
PubMed RCT 83
by phase
3phase 14phase 21phase 44no phase
by status
6completed1recruiting1not yet recruiting1terminated1unknown
references 2 papers
discussion no comments
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